http://www.flickr.com/photos/glyn_nelson/tags/senescence/ Thu, 05 Nov 2009 03:21:32 -0800 Thu, 05 Nov 2009 03:21:32 -0800 http://www.flickr.com/ http://farm1.staticflickr.com/205/buddyicons/7773270@N02.jpg?1228298975#7773270@N02 http://www.flickr.com/photos/glyn_nelson/tags/senescence/ http://www.flickr.com/photos/glyn_nelson/4076972771/ <p><a href="http://www.flickr.com/people/glyn_nelson/">glyn_nelson</a> posted a photo:</p> <p><a href="http://www.flickr.com/photos/glyn_nelson/4076972771/" title="Senescent fibroblast 1"><img src="http://farm3.staticflickr.com/2577/4076972771_37d4fbd458_m.jpg" width="240" height="180" alt="Senescent fibroblast 1" /></a></p> <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> 49sfp.jpg</p> Thu, 05 Nov 2009 03:21:32 -0800 2009-11-05T11:21:32-08:00 nobody@flickr.com (glyn_nelson) tag:flickr.com,2004:/photo/4076972771 Senescent fibroblast 1 <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> 49sfp.jpg</p> glyn_nelson 3d render human dna fluorescence mitochondria huygens rfp gfp widefield fibroblast senescence lysosomes deconvolution mrc5 http://creativecommons.org/licenses/by-nc-sa/2.0/deed.en http://www.flickr.com/photos/glyn_nelson/4077726380/ <p><a href="http://www.flickr.com/people/glyn_nelson/">glyn_nelson</a> posted a photo:</p> <p><a href="http://www.flickr.com/photos/glyn_nelson/4077726380/" title="Senescent fibroblast 2"><img src="http://farm4.staticflickr.com/3528/4077726380_8ae654f95f_m.jpg" width="240" height="187" alt="Senescent fibroblast 2" /></a></p> <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> large_sfp_043.jpg</p> Thu, 05 Nov 2009 03:21:23 -0800 2009-11-05T11:21:23-08:00 nobody@flickr.com (glyn_nelson) tag:flickr.com,2004:/photo/4077726380 Senescent fibroblast 2 <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> large_sfp_043.jpg</p> glyn_nelson 3d render human dna fluorescence mitochondria huygens rfp gfp widefield fibroblast senescence lysosomes deconvolution mrc5 http://creativecommons.org/licenses/by-nc-sa/2.0/deed.en http://www.flickr.com/photos/glyn_nelson/4077726274/ <p><a href="http://www.flickr.com/people/glyn_nelson/">glyn_nelson</a> posted a photo:</p> <p><a href="http://www.flickr.com/photos/glyn_nelson/4077726274/" title="Senescent fibroblast 3"><img src="http://farm3.staticflickr.com/2573/4077726274_f7b37c4dff_m.jpg" width="240" height="180" alt="Senescent fibroblast 3" /></a></p> <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> The nucleus floating in space, like bricks don't, is that of a poorly transfected cell, so it's mitochondria aren't labelled, and there is a small amount of red fluorescent label in it's lysosomes, so some of the brighter ones are visible.<br /> <br /> 54sfp.jpg</p> Thu, 05 Nov 2009 03:21:19 -0800 2009-11-05T11:21:19-08:00 nobody@flickr.com (glyn_nelson) tag:flickr.com,2004:/photo/4077726274 Senescent fibroblast 3 <p>Live cells expressing green fluorescent protein targetted to the mitochondria and red fluorescent protein targetted to the lysosomes, and the DNA labelled with Hoechst (blue). Images acquired as z stacks on a Leica widefield, deconvolved and rendered in Huygens (SVI).<br /> <br /> The image shows a senescent human fibroblast with a large mitochondrial network and a lot of lysosomes, both of which are typical in senescent cells.<br /> <br /> The nucleus floating in space, like bricks don't, is that of a poorly transfected cell, so it's mitochondria aren't labelled, and there is a small amount of red fluorescent label in it's lysosomes, so some of the brighter ones are visible.<br /> <br /> 54sfp.jpg</p> glyn_nelson 3d render human dna fluorescence mitochondria huygens rfp gfp widefield fibroblast senescence lysosomes deconvolution mrc5 http://creativecommons.org/licenses/by-nc-sa/2.0/deed.en